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Alexa Fluor® 647 Mouse Anti-Mouse H-2Kb Antibody (AF6-88.5)

Alexa Fluor® 647 Mouse Anti-Mouse H-2Kb Antibody (AF6-88.5)

Catalog Number: S0B80178 Application: FCM Reactivity: Ms Conjugation: Alexa Fluor® 647 Brand: Starter
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$125 USD
$125 USD
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Product Details

Product Specification


Host Mouse
Antigen H-2Kb
Synonyms H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K; H2-K1
Location Membrane
Accession P01901
Clone Number AF6-88.5
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Alexa Fluor® 647
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 0.25μg per million cells in 100μl volume Ms

Background

MHC Class I (H-2Kb) is a 45 kDa type I transmembrane glycoprotein encoded within the mouse MHC locus on chromosome 17, functioning as the murine ortholog of human HLA-A; it folds in the endoplasmic reticulum with a 12 kDa β2-microglobulin light chain to create a peptide-binding groove that preferentially accommodates 8–10-mer peptides bearing hydrophobic C-termini, and once loaded it is transported to the cell surface where it surveys CD8⁺ cytotoxic T lymphocytes for the presence of viral, tumor or self-peptides, thereby orchestrating rapid immune elimination of aberrant cells while maintaining tolerance through engagement of inhibitory receptors such as Ly49 and KIR.

Picture

FC

Flow cytometric analysis of mouse H-2Kb expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Mouse IgG2a, k Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Mouse H-2Kb Antibody (right panel) at 0.5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.