Flow cytometric analysis of Human CD81 expression on human peripheral blood cells. Human peripheral blood cells were stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD81 antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD81 Antibody (5A6)
Alexa Fluor® 647 Mouse Anti-Human CD81 Antibody (5A6)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD81 |
| Synonyms | CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28 (Tspan-28); TAPA1; TSPAN28 |
| Location | Cell membrane |
| Accession | P60033 |
| Clone Number | 5A6 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B5964 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human Peripheral Blood cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD81, also known as TAPA-1, is a member of the tetraspanin superfamily of cell surface proteins. It is a widely expressed transmembrane protein with four transmembrane domains, short amino and carboxyl cytoplasmic termini, and a large extracellular loop. CD81 plays crucial roles in various biological processes, including cell adhesion, motility, proliferation, and signal transduction. It is also involved in immune responses, forming a B cell co-receptor complex with CD19, CD21, Iga, and Igb to enhance B cell signaling. Additionally, CD81 is a receptor for hepatitis C virus (HCV) and is required for HCV infection.
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