Flow cytometric analysis of Human CD80 expression on Raji cells. Cells from the Raji (Human Burkitt's lymphoma B lymphocyte, Right) or 293T (Human embryonic kidney epithelial cell, Left) was stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Human CD80 antibody (Red line histogram) at 1.25 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD80 Antibody (S-3119)
Alexa Fluor® 647 Mouse Anti-Human CD80 Antibody (S-3119)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD80 |
| Synonyms | T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1 (B7); CD28LG; CD28LG1; LAB7 |
| Location | Cell membrane |
| Accession | P33681 |
| Clone Number | S-3119 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Raji |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD80, also known as B7-1, is a co-stimulatory molecule expressed on antigen-presenting cells (APCs) such as dendritic cells, macrophages, and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a necessary co-stimulatory signal for T cell activation. Additionally, CD80 can bind to CTLA-4, an inhibitory receptor on T cells, which serves as a negative regulatory mechanism to control the extent of the immune response. This dual functionality highlights the importance of CD80 in both initiating and modulating immune reactions, making it a key target in immunotherapy research for conditions such as cancer and autoimmune diseases.
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