Alexa Fluor® 647 Mouse Anti-Human CD73 Antibody (S-715-115)
Alexa Fluor® 647 Mouse Anti-Human CD73 Antibody (S-715-115)
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$200 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD73 |
| Synonyms | 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase, NT5E, NT5, NTE |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P21589 |
| Clone Number | S-715-115 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.2mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume |
Background
NT5E (CD73) is a surface enzyme which is expressed on multiple cells. This enzyme mediates the gradual hydrolysis of the autocrine and paracrine danger signals of ATP and ADP to anti-inflammatory adenosine. Immune suppression mediated by adenosinergic pathways is very important for maintaining immune system homeostasis. Immune suppressive functions of T regulatory cells are also dependent on CD73 expression. CD73 also occurs on anergic CD4 + T cells, thereby maintaining self-tolerance to healthy tissues as well as protecting the fetus from the mother's immune system during pregnancy.
Picture
Picture
FC
Flow cytometric analysis of Human CD73 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Alexa Fluor® 488 Mouse Anti-Human CD3 antibody and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD73 Antibody (Right panel) at 0.25 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
