Human PBMC (human peripheral blood mononuclear cells) were fixed with 4% PFA and permeabilization with the 0.1% Tween. Cells were then intracellularly stained with Alexa Fluor® 647 Mouse Anti-Human CD68 Antibody at 5μl/test / (Right panel) compared with a Mouse IgG2b, κ Isotype Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD68 Antibody (S-R657)
Alexa Fluor® 647 Mouse Anti-Human CD68 Antibody (S-R657)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Human CD68 |
| Synonyms | Macrosialin; Gp110 |
| Location | Cell membrane |
| Accession | P34810 |
| Clone Number | S-R657 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD68 is a 110-kD transmembrane glycoprotein highly expressed by human monocytes, macrophages, and cells of the monocyte-macrophage lineage, such as osteoclasts and microglia. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and the scavenger receptor family. CD68 primarily localizes to lysosomes and endosomes, with a smaller fraction circulating to the cell surface. It plays a role in promoting phagocytosis and clearing cellular debris. The protein is also involved in mediating the recruitment and activation of macrophages.
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