Flow cytometric analysis of Human CD59 expression on human peripheral blood. Human peripheral blood was stained with Alexa Fluor® 647 Mouse IgG2a Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD59 Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD59 Antibody (S-R567)
Alexa Fluor® 647 Mouse Anti-Human CD59 Antibody (S-R567)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Human CD59 |
| Synonyms | CD59 glycoprotein; 1F5 antigen; 20 kDa homologous restriction factor (HRF-20; HRF20); MAC-inhibitory protein (MAC-IP); MEM43 antigen; Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis (MIRL); Protectin |
| Location | Cell membrane |
| Accession | P13987 |
| Clone Number | S-R567 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD59, also known as protectin, is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the surface of various cells. It plays a vital role in regulating the immune system and protecting cells from the complement system. Specifically, CD59 is the only membrane-bound inhibitor of the complement membrane attack complex (MAC), an immune pore that can damage human cells. Without CD59, MAC pores formed on red blood cells result in rupture, causing chronic hemolysis and immune-related neuropathies.
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