Flow cytometric analysis of Human CD44V6 expression on A431 cells. Cells from the A431 (Human epidermoid carcinoma epithelial cell, right) or Raji (Human Burkitt's lymphoma B lymphocyte, left) was stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Human CD44v6 Antibody (red line histogram) at 1.25 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD44v6 Antibody (S-945-76)
Alexa Fluor® 647 Mouse Anti-Human CD44v6 Antibody (S-945-76)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD44v6 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | NP_001001391.1 |
| Clone Number | S-945-76 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Isotype Control | S0B5964 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | A431 |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD44 variable alternative exon 6 (CD44 V6) is a variant isoform of the CD44 gene resulting from alternative splicing of RNA. CD44 is a cell adhesion molecule that is present in various cell types and plays crucial roles in biological processes such as cell migration, proliferation, and differentiation. The CD44 V6 isoform specifically, has been implicated in tumor development and metastasis. Research has shown that CD44v6 is expressed by all colorectal cancer stem cells (CR-CSCs). The expression of CD44v6 is essential for the migration and formation of metastatic tumors, achieved through the activation of the WNT-β-catenin signaling pathway. Propelling evidence recommends that CD44v6 compared to CD44 has a more specific expression in CSCs and may have a higher diagnostic value.
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