Flow cytometric analysis of Human CD270 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Human CD270 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD270 Antibody (S-2915)
Alexa Fluor® 647 Mouse Anti-Human CD270 Antibody (S-2915)
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$250 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD270 |
| Synonyms | Tumor necrosis factor receptor superfamily member 14; Herpes virus entry mediator A (Herpesvirus entry mediator A; HveA); Tumor necrosis factor receptor-like 2 (TR2); HVEA; HVEM; TNFRSF14 |
| Location | Cell membrane |
| Accession | Q92956 |
| Clone Number | S-2915 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD270, also known as TNFRSF14 or Herpesvirus Entry Mediator (HVEM), is a member of the TNF-receptor superfamily. It is a type I transmembrane protein that plays a crucial role in mediating the entry of herpes simplex virus into cells through its interaction with the viral envelope glycoprotein D (gD). The cytoplasmic region of CD270 binds to several TRAF family members, which are involved in signal transduction pathways that activate the immune response . CD270 is widely expressed in various tissues, including blood vessels, brain, heart, and immune cells such as T cells and B cells . Its interactions with ligands like LIGHT (TNFSF14) and LTα are also important for immune regulation .
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