Flow cytometric analysis of CD244 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with PE Mouse Anti-Human CD56 Antibody and either Alexa Fluor® 647 Mouse IgG2a, k Isotype Control (left panel) or Alexa Fluor® 647 Mouse Anti-Human CD244 antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD244 Antibody (S-2854)
Alexa Fluor® 647 Mouse Anti-Human CD244 Antibody (S-2854)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD244 |
| Synonyms | Natural killer cell receptor 2B4; NK cell activation-inducing ligand (NAIL); NK cell type I receptor protein 2B4 (NKR2B4; h2B4); SLAM family member 4 (SLAMF4); Signaling lymphocytic activation molecule 4; 2B4 |
| Location | Cell membrane |
| Accession | Q9BZW8 |
| Clone Number | S-2854 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD244, also known as 2B4 or SLAMF4, is a type-I transmembrane protein belonging to the signaling lymphocytic activation molecule (SLAM) family. It is expressed on various immune cells, including NK cells, T cells, monocytes, eosinophils, myeloid-derived suppressor cells, and dendritic cells. CD244 primarily binds to CD48, another member of the SLAM family, to regulate immune responses through both stimulatory and inhibitory signals. Its cytoplasmic domain contains four immunoreceptor tyrosine-based switch motifs (ITSMs) that interact with proteins like SAP, EAT2, and phosphatases to transmit signals. CD244 plays a significant role in immune regulation and has potential as a therapeutic target in diseases such as cancer and autoimmune disorders.
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