Alexa Fluor® 647 Mouse Anti-Human CD235a/b Antibody (S-R403)
Alexa Fluor® 647 Mouse Anti-Human CD235a/b Antibody (S-R403)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD235a/b |
| Synonyms | Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GYPA, Glycophorin-B, PAS-3, SS-active sialoglycoprotein, Sialoglycoprotein delta, GYPB |
| Location | Cell membrane |
| Accession | P02724、 P06028 |
| Clone Number | S-R403 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.02mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
Glycophorin A (GYPA) and B (GYPB; this protein) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups respectively. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include the Miltenberger (Mi) complex and several isoforms of Stones (Sta); also Dantu, Sat, Henshaw (He or MNS6), Mg and deletion variants Ena, S-s-U- and Mk. Most of these are the result of gene recombinations between GYPA and GYPB.
Picture
Picture
FC
Flow cytometric analysis of Human CD235a/b expression on K562. Cells from the K562 (Human chronic myelogenous leukemia lymphoblast, Right) or MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Left) was stained with Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Mouse Anti-Human CD235a/b Antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
