Flow cytometric analysis of human CD180 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 Antibody and either Alexa Fluor® 647 Mouse IgG1, k Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD180 Antibody (right panel) at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD180 Antibody (G28-8)
Alexa Fluor® 647 Mouse Anti-Human CD180 Antibody (G28-8)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD180 |
| Synonyms | CD180 antigen; Lymphocyte antigen 64; Radioprotective 105 kDa protein; LY64; RP105 |
| Location | Cell membrane |
| Accession | Q99467 |
| Clone Number | G28-8 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD180, also known as RP105, is a cell-surface glycoprotein of the Toll-like receptor (TLR) family that consists of extracellular leucine-rich repeats and a short cytoplasmic tail, and, when paired with its accessory molecule MD-1, forms the RP105/MD-1 receptor complex that collaborates with TLR4 to recognize bacterial lipopolysaccharide and regulate innate immune signaling in B cells, leading to NF-κB activation, control of B-cell proliferation, and modulation of life-or-death decisions, while its dysfunction is implicated in diseases such as chronic lymphocytic leukemia and Kawasaki disease.
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