Flow cytometric analysis of CD144 expression on HUVEC (human umbilical vein endothelial cell). Cells from HUVEC were stained with either Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (black line histogram) or SDT Alexa Fluor® 647 Mouse Anti-Human CD144 Antibody (red line histogram) at 5 μl/test. Cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human CD144 Antibody (S-3026)
Alexa Fluor® 647 Mouse Anti-Human CD144 Antibody (S-3026)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD144 |
| Synonyms | Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5 |
| Location | Cell membrane, Cell junction |
| Accession | P33151 |
| Clone Number | S-3026 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | HUVEC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation .
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