Flow cytometric analysis of U-937 (Human histiocytic lymphoma monocyte, left) / THP-1 (Human monocytic leukemia monocyte, right) were stained with Alexa Fluor® 647 Mouse IgG1, κ Isotype Control (black line histogram) or SDT Alexa Fluor® 647 Mouse anti-Human CD135(red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Negative control: U-937
Alexa Fluor® 647 Mouse Anti-Human CD135 Antibody (S-3176)
Alexa Fluor® 647 Mouse Anti-Human CD135 Antibody (S-3176)
Price:
Regular price
$170 USD
Regular price
Sale price
$170 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD135 |
| Synonyms | Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2 (FLK-2); Fms-like tyrosine kinase 3 (FLT-3); Stem cell tyrosine kinase 1 (STK-1); FLK2; STK1; FLT3 |
| Location | Membrane |
| Accession | P36888 |
| Clone Number | S-3176 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | THP-1 |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD135, also known as FLT3 (Fms-like tyrosine kinase 3), is a class III receptor tyrosine kinase that plays a critical role in the regulation of hematopoiesis by promoting the survival, proliferation, and differentiation of hematopoietic stem and progenitor cells upon binding to its ligand FLT3L; upon activation, CD135 undergoes dimerization and trans-phosphorylation, triggering downstream signaling cascades such as RAS/MAPK and PI3K/AKT pathways, which are essential for lymphoid lineage development and dendritic cell differentiation, while mutations—especially internal tandem duplications (FLT3-ITD)—lead to constitutive kinase activity, contributing to oncogenesis, particularly in acute myeloid leukemia (AML), where it is associated with poor prognosis and targeted by specific kinase inhibitors.
Picture
Picture
FC
