2e5 of transient transfected anti- CD30 ScFv CAR-293 cells were stained with 0.01ug Alexa Fluor 647-Labeled CD30/TNFRSF8 His Tag Protein, Human, (Cat. No. UA011322) and unlable respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity.
2e5 of transient transfected anti- CD30 ScFv CAR-293 cells were stained with isotype and Whitlow/218 Linker-Alexa Fluor® 488 (Fig. D and E). Alexa Fluor® 488 signal was used to evaluate the binding activity.
Alexa Fluor 647-Labeled CD30/TNFRSF8 His Tag Protein, Human
Alexa Fluor 647-Labeled CD30/TNFRSF8 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | TNFRSF8,CD30,D1S166E,Ki-1 |
| Accession | P28908 |
| Amino Acid Sequence | Phe19-Lys379 with His Tag at C-terminal |
| Expression System | HEK293 |
| Molecular Weight | 72-89kDa (Reducing) |
| Conjugation | Alexa Fluor® 647 |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | PBS 7.4 |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1、Feghali-Bostwick C A. et al. (2008) Autoantibodies in patients with chronic obstructive pulmonary disease. American Journal of Respiratory and Critical Care Medicine. 177(2): 156-163. |
Background
CD30, a member of the tumor necrosis factor receptor superfamily, also known as Ki-1, is a kind of type I transmembrane glycoprotein. The CD30 protein is composed of 595 amino acids, and the cytosolic and transmembrane region in the protein are composed of 188 amino acids and 24 amino acids, respectively. The extracellular region is the leading peptide of 18 amino acids, followed by 365 amino acids. CD30 is widely expressed in viral-infected lymphocytes and lymphoma cells and activated T cells. Moreover, CD30 is also expressed in alveolar macrophages, epidermal cells, activated NK cells, decidual cells, resting B cells, granulocytes, thymocytes, and medulla cells. The bind of CD30 and CD30L induces the production of cytokine, which promotes cell proliferation, differentiation, cell growth, stagnation, and death. Binding of CD30 with its receptor ligand activates TNF receptor-associated factor (TRAF)2 and TRAF5, initiating signal transduction pathways that can lead either to proliferation or apoptosis. CD30 induced apoptosis may lead to the self-regression seen in lymphomatoid papulosis lesions, whereas proliferation may result in anaplastic large-cell lymphoma (ALCL). CD30 ligation leads to nuclear factor-kappa B (NFκB) and/or mitogen-activated protein kinase (MAPK) pathways, ultimately leading to survival of neoplastic cells. CD30 can serve both as a diagnostic marker for lymphocyte malignancies and as a target for therapy.
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