Flow cytometric analysis of mouse splenocytes labeling Alexa Fluor® 647 Human IgG4 S228P, kappa isotype control at 1/20 dilution (1 μg) / (Right panel) compared with a unlable Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Human IgG4 S228P, kappa isotype control (S-3185)
Alexa Fluor® 647 Human IgG4 S228P, kappa isotype control (S-3185)
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Product Details
Product Details
Product Specification
| Clone Number | S-3185 |
| Antibody Type | Recombinant mAb |
| Application | FCM, ICFCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
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FC
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labeling Alexa Fluor® 647 Human IgG4 S228P, kappa isotype control at 1/20 dilution (1 μg) / (Right panel) compared with a unlable Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
