Flow cytometric analysis of Mouse/Rat CD61 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow cells were stained with PE Rat Anti-Mouse TER-119 Antibody and either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat CD61 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat CD61 Antibody (S-R656)
Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat CD61 Antibody (S-R656)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD61 |
| Synonyms | Integrin beta-3; Platelet membrane glycoprotein IIIa (GPIIIa); Itgb3 |
| Location | Cell membrane, Synapse |
| Accession | O54890 |
| Clone Number | S-R656 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms, Rt |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms, Rt |
Background
CD61, also known as integrin β3, is a 90-92 kDa type I transmembrane glycoprotein that dimerizes with integrin αIIb (CD41) to form the platelet fibrinogen receptor GPIIb/IIIa or with integrin αV (CD51) to form the vitronectin receptor, and plays a pivotal role in platelet aggregation, clot retraction, angiogenesis, and the adhesion and migration of osteoclasts, smooth muscle cells and tumor cells via recognition of RGD motifs in ligands such as fibrinogen, fibronectin, von Willebrand factor, vitronectin and thrombospondin.
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