Flow cytometric analysis of CD29 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow was stained with Pacific Blue™ Rat Anti-Mouse CD45R/B220 Antibody and either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat CD29 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat CD29 Antibody (S-R675)
Alexa Fluor® 647 Armenian hamster Anti-Mouse/Rat CD29 Antibody (S-R675)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD29 |
| Synonyms | Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; Itgb1 |
| Location | Endosome, Cell membrane |
| Accession | P09055、P49134 |
| Clone Number | S-R675 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.02mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD29, also known as integrin beta-1 (ITGB1), is a crucial cell surface receptor involved in cell adhesion, migration, and signaling. It is a member of the integrin family and is encoded by the ITGB1 gene, located on human chromosome 10p11.2. CD29 forms complexes with various integrin alpha subunits to interact with extracellular matrix (ECM) proteins such as collagen, laminin, and fibronectin. This protein plays a significant role in cellular processes including “inside-out” and “outside-in” signaling pathways, which are essential for cell survival and metastasis. Additionally, CD29 is highly expressed in various cell types and is implicated in multiple diseases, including cancer progression and immune regulation.
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