Flow cytometric analysis of Mouse CTLA-4 (CD152) expression on Concanavalin A stimulated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were either not stimulated (left panel) or stimulated 72h with 2.5μg/ml Concanavalin A (right panel) and then were stained with SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse CTLA-4 (CD152) Antibody at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Armenian hamster Anti-Mouse CTLA-4 (CD152) Antibody (UC10-4F10-11)
Alexa Fluor® 647 Armenian hamster Anti-Mouse CTLA-4 (CD152) Antibody (UC10-4F10-11)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CTLA-4 (CD152) |
| Synonyms | Cytotoxic T-lymphocyte protein 4; Cd152; Ctla4 |
| Location | Cell membrane |
| Accession | P09793 |
| Clone Number | UC10-4F10-11 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | ConA treated with C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4), also known as CD152, is a transmembrane protein expressed on the surface of activated T cells. It plays a role in the negative regulation of the immune response and is one of the key targets in immune checkpoint therapy. CTLA-4 shares the same ligands, B7 molecules (including CD80 and CD86), with CD28; however, unlike CD28, the binding of CTLA-4 to B7 molecules inhibits T cell activation. This inhibitory effect helps prevent excessive immune responses, but in the tumor microenvironment, tumor cells may exploit CTLA-4 to evade immune surveillance and attack.
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