Flow cytometric analysis of Mouse CD80 expression on C57BL/6 mouse splenocytes treated with LPS. C57BL/6 mouse splenocytes (Green line histogram) or treated with LPS (500ng/ml, 3d) (Red line histogram) were stained with SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse CD80 Antibody at 1.25 μl/test compared with a Alexa Fluor® 647 Armenian hamster IgG (Black line histogram) Isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Armenian hamster Anti-Mouse CD80 Antibody (S-R520)
Alexa Fluor® 647 Armenian hamster Anti-Mouse CD80 Antibody (S-R520)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD80 |
| Synonyms | T-lymphocyte activation antigen CD80; Activation B7-1 antigen (B7); B7; Cd80 |
| Location | Cell membrane |
| Accession | Q00609 |
| Clone Number | S-R520 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | LPS treated C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
CD80, also known as B7-1，is a co - stimulatory molecule expressed on antigen - presenting cells (APCs) such as dendritic cells, macrophages and B cells. It plays a crucial role in the immune response by interacting with CD28 on T cells to provide a co - stimulatory signal necessary for T cell activation, thereby promoting T cell proliferation and differentiation. Additionally, CD80 can also bind to CTLA - 4，an inhibitory receptor on T cells, which acts as a negative regulator of T cell activation. This binding can inhibit the immune response and maintain immune tolerance. Moreover, CD80 - CD28 interaction is involved in the regulation of various immune - related processes, including the formation of immunological synapses and the regulation of cytokine production.
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FC
Multiple Flow Analysis
Flow cytometric analysis of Mouse CD80 expression on C57BL/6 mouse splenocytes treated with LPS. C57BL/6 mouse splenocytes (Green line histogram) or treated with LPS (500ng/ml, 3d) (Red line histogram) were stained with SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse CD80 Antibody at 1.25 μl/test compared with a Alexa Fluor® 647 Armenian hamster IgG (Black line histogram) Isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
