Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling SDT Alexa Fluor® 594 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT CD38 antibody at 0.1 μg/test (S0B0128) (Right) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
Host | Rabbit |
Clone Number | S-R058 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | FCM |
Purification | Protein A |
Concentration | 0.2 mg/ml |
Conjugation | Alexa Fluor® 594 |
Physical Appearance | Liquid |
Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labeling SDT Alexa Fluor® 594 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT β-tubulin antibody at 0.1 μg/test (S0B0133) (Right) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
