Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling SDT Alexa Fluor® 594 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT CD38 antibody at 0.1 μg/test (S0B0128) (Right) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-R058 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 594 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest. Will delete after testing.
Picture
Picture
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labeling SDT Alexa Fluor® 594 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT β-tubulin antibody at 0.1 μg/test (S0B0133) (Right) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
