Flow cytometric analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) labelling Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody at 1/20 dilution (1μg) / (Black) compared with CD38 antibody / (Red), and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 555 was used as the secondary antibody.
Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody (S-R058)
Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody (S-R058)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-R058 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, FCM, IF |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) labelling Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody at 1/20 dilution (1μg) / (Black) compared with β-tubulin (Alexa Fluor® 555 Conjugate) antibody at 1/200 (1 μg) dilution (S0B0135) / (Red) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Immunocytochemistry
ICC shows negative staining in HeLa cells. Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody was used at 1/5 dilution and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows negative staining in NIH/3T3 cells. Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody was used at 1/5 dilution and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Immunofluorescence
IF shows negative staining in paraffin-embedded human prostatic cancer. Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody was used at 1/5 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded human breast cancer. Alexa Fluor® 555 Rabbit IgG Isotype Control Antibody was used at 1/5 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
