Flow cytometric analysis of CD44 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with Alexa Fluor® 488 Rat IgG2b, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse/Human CD44 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse/Human CD44 Antibody (IM7)
Alexa Fluor® 488 Rat Anti-Mouse/Human CD44 Antibody (IM7)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD44 |
| Synonyms | CD44 antigen; CDw44; Epican; Extracellular matrix receptor III (ECMR-III); GP90 lymphocyte homing/adhesion receptor; HUTCH-I; LHR; MDU2; MDU3; MIC4 |
| Location | Cell membrane |
| Accession | P16070、P15379 |
| Clone Number | IM7 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Hu, Ms |
| Positive Sample | C57BL/6 mouse bone marrow, Human peripheral blood cells |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Hu, Ms |
Background
CD44 is a multifunctional transmembrane glycoprotein that functions as a major adhesion molecule for hyaluronan and other extracellular matrix components, playing critical roles in cell migration, proliferation, differentiation, and survival; it is expressed in numerous isoforms generated through alternative splicing, with its standard form (CD44s) and variant isoforms (CD44v) exhibiting distinct tissue distributions and functional properties, and its dysregulated expression has been implicated in various pathological processes including inflammation, autoimmune diseases, and cancer progression where it contributes to tumor cell invasion, metastasis, and resistance to apoptosis.
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Flow cytometric analysis of CD44 expression on human peripheral blood cells. Human peripheral blood cells were stained with Alexa Fluor® 488 Rat IgG2b, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse/Human CD44 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
