Flow cytometric analysis of Mouse TCR Vα2 expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either Rat IgG2a, L Isotype Control (Left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse TCR Vα2 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse TCR Vα2 Antibody (S-R698)
Alexa Fluor® 488 Rat Anti-Mouse TCR Vα2 Antibody (S-R698)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | TCR Vα2 |
| Synonyms | T-cell receptor alpha chain constant; Trac |
| Location | Cell membrane |
| Accession | P01849 |
| Clone Number | S-R698 |
| Antibody Type | Rat mAb |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
TCR Vα2 is a distinct TCR subfamily found in mice with a, b, and c haplotypes. It is a variable region of the T cell receptor (TCR) that plays a crucial role in recognizing antigens bound to major histocompatibility complex (MHC) molecules. The TCR Vα2 subfamily is often expressed in both CD4 and CD8 subsets of T cells, although its expression can be skewed to CD8 cells when combined with certain TCR β-chain transgenes. It is also involved in the positive selection of CD8+ T cells in the thymus.
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