Flow cytometric analysis of Ly-6A/E expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow cells were stained with APC Rat Anti-Mouse CD117 Antibody and either Alexa Fluor®488 Rat IgG2a, k Isotype Control (left panel) or Alexa Fluor®488 Rat Anti-Mouse Ly-6A/E Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse Ly-6A/E Antibody (D7)
Alexa Fluor® 488 Rat Anti-Mouse Ly-6A/E Antibody (D7)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly-6A/E |
| Synonyms | Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1 (SCA-1); T-cell-activating protein (TAP); Ly6; Ly6a |
| Location | Cell membrane |
| Accession | P05533、Q64253 |
| Clone Number | D7 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Isotype Control | S0B5537 |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
Ly-6A/E, also known as Sca-1 (stem cell antigen-1), is an 18-kDa glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the murine Ly-6 multigene family, predominantly expressed on multipotent hematopoietic stem cells in adult mouse bone marrow and fetal liver, and is widely used as a marker for isolating these stem cells; beyond its stem cell association, Ly-6A/E is upregulated on activated T and B lymphocytes and appears to participate in T-cell receptor-mediated activation, myoblast proliferation and differentiation, bone formation, and interferon-induced immune responses, with expression levels varying between mouse strains carrying different Ly-6 haplotypes.
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