Flow cytometric analysis of Ki-67 expression on ConA treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes treated 72h with ConA were stained with Alexa Fluor® 488 Rat IgG2a, k Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse Ki-67 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse Ki-67 Antibody (S-R595)
Alexa Fluor® 488 Rat Anti-Mouse Ki-67 Antibody (S-R595)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ki67 |
| Synonyms | Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog (Antigen KI-67 homolog; Antigen Ki67 homolog); Mki67 |
| Location | Nucleus |
| Accession | E9PVX6 |
| Clone Number | S-R595 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Isotype Control | S0B5537 |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | ConA treated C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1μg per million cells in 100μl volume | Ms |
Background
Ki67 protein is a non-histone nuclear protein encoded by the MKI67 gene, widely used as a proliferation marker in pathology. It is expressed during all active phases of the cell cycle—G1, S, G2, and M—but is absent in quiescent (G0) cells. Ki67 has multiple molecular functions, including forming the perichromosomal layer to prevent chromosome aggregation during mitosis and organizing heterochromatin. It also facilitates chromosome attachment to the mitotic spindle and individual chromosome mobility. In addition to its role in cell division, Ki67 is a valuable prognostic and predictive indicator in cancer diagnosis, with its expression levels correlating with tumor cell proliferation rates.
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