Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized C57BL/6 mouse splenocytes, Stimulated 6 hours with Stimulation Cocktail labelled with Alexa Fluor® 488 Rat IgG2b, k Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse IL-2 Antibody at 2 μl/test (right panel). Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse IL-2 Antibody (JES6-5H4)
Alexa Fluor® 488 Rat Anti-Mouse IL-2 Antibody (JES6-5H4)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-2 |
| Synonyms | Interleukin-2; T-cell growth factor (TCGF); Il-2; Il2 |
| Location | Secreted |
| Accession | P04351 |
| Clone Number | JES6-5H4 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Isotype Control | S0B5536 |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes stimulated for 6h with the cell stimulation cocktail |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1μg per million cells in 100μl volume | Ms |
Background
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is primarily produced by activated T cells and has multiple functions. IL-2 promotes the proliferation and differentiation of T cells, enhancing their ability to fight infections and tumors. It also stimulates the growth and activity of natural killer (NK) cells, which are important for immune defense. Additionally, IL-2 can regulate the activity of regulatory T cells (Tregs), helping to maintain immune tolerance and prevent autoimmune diseases. In clinical settings, IL-2 has been used to treat certain cancers by boosting the immune response, although its use is limited due to potential side effects. Its discovery and research have significantly advanced our understanding of the immune system and provided new strategies for immunotherapy.
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