Flow cytometric analysis of Mouse CD104 expression on bEnd.3 (Mouse brain endothelioma). bEnd.3 were stained with Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse CD104 Antibody (right panel) at 2 μl/test. The bivariate pseudocolor density plot shows CD104 expression derived from CD4- gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 488 Rat Anti-Mouse CD104 Antibody (S-R716)
Alexa Fluor® 488 Rat Anti-Mouse CD104 Antibody (S-R716)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Integrin beta 4 |
| Synonyms | Integrin beta-4; CD104; Itgb4 |
| Location | Cell membrane |
| Accession | A2A863 |
| Clone Number | S-R716 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Isotype Control | S0B5537 |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | bEnd.3 |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1μg per million cells in 100μl volume | Ms |
Background
Integrin beta 4 (ITGB4) is a key subunit of the integrin family, primarily functioning as a receptor for laminins and often associating with the alpha 6 subunit to form the α6β4 integrin complex. This transmembrane glycoprotein receptor plays a crucial role in mediating cell-matrix adhesion and signaling, which regulates gene expression, cell growth, and migration. ITGB4 is particularly important in maintaining the stability of hemidesmosomes, which anchor epithelial cells to the basement membrane. Additionally, its expression and activity are implicated in various biological processes, including wound healing, tissue integrity, and cancer progression. Abnormal mutations or dysregulation of ITGB4 have been associated with diseases such as epidermolysis bullosa with pyloric atresia.
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