Flow cytometric analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) labeling SDT Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT CD38 antibody at 1 μg/test (S0B0092) (Ritht) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody (S-R058)
Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody (S-R058)
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$110 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Clone Number | S-R058 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
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FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labeling SDT Alexa Fluor® 488 Rabbit IgG Isotype Control Antibody at 1 μg/test (Left) / (Red line histogram) compared with SDT β-tubulin antibody at 1 μg/test dilution (S0B0132) (Right) / (Red line histogram) compared with a Rabbit monoclonal IgG (Black line histogram) isotype control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
