Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte, left) / A431 (Human epidermoid carcinoma epithelial cell, right) stained with Alexa Fluor® 488 Mouse IgG Isotype Control (black line histogram) and SDT Alexa Fluor® 488 Rabbit Anti-Human CD44 Antibody (red line histogram) at 5 μl/test. Cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Negative control: Raji
Alexa Fluor® 488 Rabbit Anti-Human CD44 Antibody (S-944-25)
Alexa Fluor® 488 Rabbit Anti-Human CD44 Antibody (S-944-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD44 |
| Synonyms | CD44 antigen; CDw44; Epican; Extracellular matrix receptor III (ECMR-III); GP90 lymphocyte homing/adhesion receptor; HUTCH-I; LHR; MDU2; MDU3; MIC4 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16070 |
| Clone Number | S-944-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Isotype Control | S0B0161 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | A431 |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD44 is a multifunctional transmembrane glycoprotein that functions as a major adhesion molecule for hyaluronan and other extracellular matrix components, playing critical roles in cell migration, proliferation, differentiation, and survival; it is expressed in numerous isoforms generated through alternative splicing, with its standard form (CD44s) and variant isoforms (CD44v) exhibiting distinct tissue distributions and functional properties, and its dysregulated expression has been implicated in various pathological processes including inflammation, autoimmune diseases, and cancer progression where it contributes to tumor cell invasion, metastasis, and resistance to apoptosis.
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