Alexa Fluor® 488 Mouse Anti-Rat TCR γ/δ Antibody (V65)

TCR γ/δ is a heterodimeric receptor—composed of one γ and one δ chain, each with a variable (V) and constant (C) immunoglobulin-like domain plus a short cytoplasmic tail—whose extracellular V regions recognize phosphoantigens, stress molecules or butyrophilin family ligands without MHC restriction, while its membrane-proximal C regions associate with CD3δε, γε and ζζ signaling modules; upon ligand binding the γ/δ chains undergo conformational change that exposes CD3 ITAMs to Lck-mediated phosphorylation, recruiting ZAP-70 and triggering a Vav1–PLC-γ1 cascade that elevates intracellular Ca²⁺, activates PKC-θ, NFAT, NF-κB and AP-1, and thereby drives γδ T-cell cytotoxicity, cytokine release and tumor lysis.