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Alexa Fluor® 488 Mouse Anti-Mouse H-2Kd Antibody (SF1-1.1)

Alexa Fluor® 488 Mouse Anti-Mouse H-2Kd Antibody (SF1-1.1)

Catalog Number: S0B8642 Application: FCM Reactivity: Ms Conjugation: Alexa Fluor® 488 Brand: Starter
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$88 USD
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Product Details

Product Specification


Host Mouse
Antigen H-2Kd
Synonyms H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K; H2-K1
Location Membrane
Accession P01902
Clone Number SF1-1.1
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Ms
Positive Sample BALB/c mouse splenocytes
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 488
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

H-2Kd is a murine MHC class I molecule expressed by BALB/c mice that presents endogenous 9-mer peptides—anchored by Tyr at position 2 and a hydrophobic residue at position 9—to CD8⁺ cytotoxic T cells, thereby initiating adaptive immune responses; its 2.6-Å crystal structure reveals a peptide-binding groove with five deep pockets (A–F) that impose an allele-specific motif, and it pairs non-covalently with β2-microglobulin for stability.

Picture

FC

Flow cytometric analysis of Mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Mouse H-2Kd Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.