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Alexa Fluor® 488 Mouse Anti-Mouse CD3ε Antibody (S-R384)

Alexa Fluor® 488 Mouse Anti-Mouse CD3ε Antibody (S-R384)

Catalog Number: S0B8399 Application: FCM Reactivity: Mouse Conjugation: Alexa Fluor 488 Brand: Starter
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Product Details

Product Specification


Host Mouse
Antigen CD3ε
Synonyms T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; Cd3e
Location Cell membrane
Accession P22646
Clone Number S-R384
Antibody Type Mouse mAb
Isotype IgG1,k
Application FCM
Reactivity Ms
Positive Sample C57BL/6 mouse splenocytes
Purification Protein G
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 488
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Ms

Background

CD3ε protein, also known as CD3E, is a crucial component of the T-cell receptor (TCR) complex, which is essential for T-cell activation and function. It is a single-pass type I membrane glycoprotein that contains one Ig-like domain and one immunoreceptor tyrosine-based activation motif (ITAM) domain. CD3ε, along with CD3γ, CD3δ, and CD3ζ, forms the TCR-CD3 complex, which plays a vital role in coupling antigen recognition to intracellular signal-transduction pathways. This protein is essential for the proper development of T-cells and is involved in the internalization and downregulation of the TCR-CD3 complex on the cell surface. Defects in the CD3ε gene can lead to severe immunodeficiency and have been linked to a susceptibility to type I diabetes in women.

Picture

FC

flow cytometric analysis of Mouse CD3ε expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD19 antibody and either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Mouse CD3ε Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.