Flow cytometric analysis of Mouse CD20 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD3 Antibody and either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Left panel) or SDT Alexa Fluor® 488 Mouse Anti-Mouse CD20 Antibody (18B12)(Right panel) at 1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Mouse CD20 Antibody (18B12)
Alexa Fluor® 488 Mouse Anti-Mouse CD20 Antibody (18B12)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD20 |
| Synonyms | B-lymphocyte antigen CD20, B-cell differentiation antigen Ly-44, Lymphocyte antigen 44, Membrane-spanning 4-domains subfamily A member 1, Ms4a1, Ly-44, Ms4a2 |
| Accession | P19437 |
| Clone Number | 18B12 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 0.2mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume |
Background
Mouse CD20 is a B-cell-specific transmembrane protein with a molecular weight of 33~37 kDa, also known as B-lymphocyte antigen, B1, and Bp35. It plays a crucial role in intracellular calcium regulation and B-cell activation, which is essential for optimal B-cell immune responses against T-independent antigens. During the transition of pre-B cells to immature B cells in the bone marrow, CD20 is first expressed under the combined induction of CD19 and IgM. Subsequently, as B cells mature, the expression level of CD20 gradually increases, and almost all mature B cells express a certain level of CD20. However, it is noteworthy that plasma cells or plasmablasts do not express CD20.
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