Flow cytometric analysis of human Siglec-8 expression on human peripheral blood cells. Human peripheral blood cells were stained with Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human Siglec-8 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Human Siglec-8 Antibody (S-4479)
Alexa Fluor® 488 Mouse Anti-Human Siglec-8 Antibody (S-4479)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Siglec-8 |
| Synonyms | Sialic acid-binding Ig-like lectin 8; Siglec-8; Sialoadhesin family member 2 (SAF-2); SAF2; SIGLEC8 |
| Location | Membrane |
| Accession | Q9NYZ4 |
| Clone Number | S-4479 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood cells |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
Siglec-8 is a CD33-related cell-surface lectin selectively expressed on human eosinophils and, at lower density, on mast cells and basophils; it carries an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif that, upon engagement by sialylated glycan ligands, recruit SHP-1/2 phosphatases to deliver a potent inhibitory signal that suppresses FcεRI- or cytokine-driven activation, survival and mediator release, thereby serving as a key “off-switch” for allergic inflammation and making it an attractive therapeutic target for eosinophilic asthma, chronic urticaria and other IgE-mediated diseases.
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