Flow cytometric analysis of Human CD31 expression on Human PBMC (human peripheral blood mononuclear cells). Human PBMC was stained with Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (Left Panel) and SDT Alexa Fluor® 488 Mouse Anti-Human CD31 antibody (Right Panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Human CD31 Antibody (S-934-6)
Alexa Fluor® 488 Mouse Anti-Human CD31 Antibody (S-934-6)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD31 |
| Synonyms | Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; PECAM1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16284 |
| Clone Number | S-934-6 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a 130 kDa transmembrane glycoprotein of the immunoglobulin superfamily composed of six extracellular Ig-like domains, a single transmembrane region, and a cytoplasmic tail with multiple phosphorylation sites, and it is constitutively expressed on endothelial cells, platelets, leukocytes, and hematopoietic stem cells, where it mediates homophilic and heterophilic cell–cell adhesion, facilitates leukocyte transendothelial migration, regulates vascular permeability, and plays an essential role in developmental and pathological angiogenesis by promoting endothelial tube formation and neovessel stabilization.
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