Flow cytometric analysis of Human CD25 expression on PHA treated Human PBMC (human peripheral blood mononuclear cells). Human PBMC treated 48 hours with 10μg/ml PHA (Right panel) or untreated (Left panel) were stained with Brilliant Violet 421™ Mouse Anti-Human CD4 Antibody and SDT Alexa Fluor®488 Mouse Anti-Human CD25 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Human CD25 Antibody (S-3536)
Alexa Fluor® 488 Mouse Anti-Human CD25 Antibody (S-3536)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD25 |
| Synonyms | Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; IL2RA |
| Location | Membrane |
| Accession | P01589 |
| Clone Number | S-3536 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | PHA stimulated human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD25, also known as IL2RA, is the alpha chain of the interleukin-2 receptor, a heterotrimeric protein complex that also includes beta (IL2RB) and the common gamma chain (IL2RG). It is a membranous glycoprotein primarily expressed on activated T lymphocytes and plays a crucial role in immune homeostasis by interacting with interleukin-2, a pleiotropic cytokine. CD25 is involved in the proliferation of T and B lymphocytes and is essential for the assembly of the high-affinity interleukin-2 receptor. In addition to its role in the immune system, CD25 is also expressed on certain non-immune cells and has been identified as a potential therapeutic target in cancers.
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