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Alexa Fluor® 488 Mouse Anti-Human CD199 Antibody (S-3012)

Alexa Fluor® 488 Mouse Anti-Human CD199 Antibody (S-3012)

Catalog Number: S0B5755 Application: FCM Reactivity: Human Conjugation: Alexa Fluor® 488 Brand: Starter
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$196.00 SGD
$196.00 SGD
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Product Details

Product Specification


Host Mouse
Antigen CD199
Synonyms C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR-9; G-protein coupled receptor 28; GPR-9-6; CDw199; GPR28; CCR9
Location Cell membrane
Accession P51686
Clone Number S-3012
Antibody Type Mouse mAb
Isotype IgG2a,k
Application FCM
Reactivity Hu
Positive Sample MOLT-4
Purification Protein A
Concentration 0.2 mg/ml
Conjugation Alexa Fluor® 488
Physical Appearance Liquid
Storage Buffer

PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

Dilution


application dilution species
FCM 5μl per million cells in 100μl volume Hu

Background

CD199 (also designated CCR9) is a ~42 kDa seven-transmembrane G-protein-coupled chemokine receptor that binds the chemokine CCL25 (TECK), is strongly expressed in thymic cortex and small intestinal epithelium, and is highly expressed on CD4+CD8+ double-positive thymocytes and naive CD8+ T cells, as well as on small intestinal B cells, subsets of memory CD4+/CD8+ T cells, and dendritic cells, thereby directing T-cell development in the thymus and lymphocyte homing to gut-associated tissues via the CCR9/CCL25 axis, with dysregulation implicated in inflammatory bowel disease and celiac disease .

Picture

FC

Flow cytometric analysis of Human CD199 expression on MOLT-4 cells. Cells from the MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Right) or Raji (Human Burkitt's lymphoma B lymphocyte, Left) was stained with either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Human CD199 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.