Product Specification
| Host |
Mouse |
| Antigen |
CD16a |
| Synonyms |
Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; FcRIII; FcRIIIa; FcgammaRIIIA; FcR-10; IgG Fc receptor III-2; FCGR3A; FCG3; FCGR3; IGFR3 |
| Immunogen |
Synthetic Peptide |
| Location |
Secreted, Cell membrane |
| Accession |
P08637 |
| Clone Number |
S-564-39 |
| Antibody Type |
Mouse mAb |
| Isotype |
IgG1,k |
| Application |
FCM |
| Reactivity |
Hu |
| Positive Sample |
Human PBMC |
| Purification |
Protein G |
| Concentration |
0.05mg/ml |
| Conjugation |
Alexa Fluor® 488 |
| Physical Appearance |
Liquid |
| Storage Buffer |
PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage |
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application |
dilution |
species |
| FCM |
5 μl per million cells in 100μl volume |
Hu |
Background
CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), which participate in signal transduction. The most well-researched membrane receptor implicated in triggering lysis by NK cells, CD16 is a molecule of the immunoglobulin superfamily (IgSF) involved in antibody-dependent cellular cytotoxicity (ADCC). It can be used to isolate populations of specific immune cells through fluorescent-activated cell sorting (FACS) or magnetic-activated cell sorting, using antibodies directed towards CD16. CD16 plays a significant role in early activation of natural killer (NK) cells following vaccination. In addition, CD16 downregulation represents a possible way to moderate NK cell responses and maintain immune homeostasis in both T cell and antibody-dependent signaling pathways.
