Flow cytometric analysis of Foxp3 / Transcription Factor Staining Buffer Set fixed and permeabilized Human PBMC (human peripheral blood mononuclear cells) were stained with Alexa Fluor® 488 Mouse IgG1, k Isotype Control (black line histogram) or SDT Alexa Fluor® 488 Mouse Anti Human Aiolos antibody (red line histogram) at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Mouse Anti-Human Aiolos Antibody (S-3264)
Alexa Fluor® 488 Mouse Anti-Human Aiolos Antibody (S-3264)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Aiolos |
| Synonyms | Zinc finger protein Aiolos; Ikaros family zinc finger protein 3; ZNFN1A3; IKZF3 |
| Location | Cytoplasm, Nucleus |
| Accession | Q9UKT9 |
| Clone Number | S-3264 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | ICFCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
Aiolos (IKZF3) is a lymphoid-restricted zinc-finger transcription factor that partners with Ikaros to control B-cell differentiation, proliferation and apoptosis by remodeling chromatin and directly regulating genes such as BCL2 and BCL6; its expression peaks in peripheral blood leukocytes, spleen and thymus, is induced by TGFB1 and dioxin, and its loss-of-function or deletion is linked to B-cell lymphoma, chronic lymphocytic leukemia and multiple myeloma, while over-expression promotes migration and invasion of lung cancer cells and is being exploited as a target for PROTAC degraders.
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