C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Mouse Anti-Human CD4 Antibody and fixed and permeabilized with the eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent. The cells were then stained with either Alexa Fluor® 488 Armenian hamster IgG Isotype Control (left panel) or SDT Alexa Fluor® 488 Armenian hamster Anti-Mouse/Human Helios Antibody (right panel) at 0.5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 488 Armenian Hamster Anti-Mouse/Human Helios Antibody (22F6)
Alexa Fluor® 488 Armenian Hamster Anti-Mouse/Human Helios Antibody (22F6)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | Helios |
| Synonyms | Zinc finger protein Helios; HELIOS; ZNFN1A2; IKZF2 |
| Location | Nucleus |
| Accession | Q9UKS7、P81183 |
| Clone Number | 22F6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICFCM |
| Reactivity | Hu, Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 0.25μg per million cells in 100μl volume | Hu, Ms |
Background
Helios (encoded by the IKZF2 gene) is a member of the Ikaros family of transcription factors and contains multiple zinc finger domains that facilitate DNA binding and the formation of homodimers or heterodimers. It is predominantly expressed in T cells, regulatory T cells (Tregs), certain B cell subsets, and hematopoietic and epithelial tissues. Helios plays a crucial role in T cell activation, proliferation, and the stability and function of Tregs. Additionally, Helios has been identified as a potential biomarker in autoimmune diseases and may influence tumor development by regulating gene expression and modulating cell differentiation and proliferation.
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