Aldolase A Recombinant Rabbit mAb (S-4007-23)

Catalog Number: S0B6965 Application: WB, IHC-P, ICC Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Aldolase A
Synonyms	Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDA; ALDOA
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	P04075
Clone Number	S-4007-23
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	HeLa, HEK-293, HepG2, MCF7, NIH/3T3, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu, Ms

Background

Aldolase A, also known as fructose-bisphosphate aldolase A or ALDOA, is a crucial glycolytic enzyme primarily expressed in skeletal muscle and erythrocytes that catalyzes the reversible cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P), thereby playing a pivotal role in both glycolysis and gluconeogenesis. As a homotetrameric protein belonging to the class I aldolase family, it forms a Schiff base intermediate with its substrate via a specific lysine residue in the active site, distinguishing it mechanistically from class II aldolases that rely on metal ions. Beyond its canonical metabolic function, Aldolase A has been identified as a moonlighting protein involved in various non-glycolytic processes, including actin cytoskeleton organization, cell motility, and mRNA stability, and its dysregulation or overexpression is frequently associated with several pathological conditions, particularly various types of cancer where it supports the heightened energy demands of proliferating tumor cells through the Warburg effect, making it a potential biomarker and therapeutic target in oncology and metabolic disorders.

Picture

Western Blot

WB result of Aldolase A Recombinant Rabbit mAb
Primary antibody: Aldolase A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa

WB result of Aldolase A Recombinant Rabbit mAb
Primary antibody: Aldolase A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa

WB result of Aldolase A Recombinant Rabbit mAb
Primary antibody: Aldolase A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat skeletal muscle lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa

WB result of Aldolase A Recombinant Rabbit mAb
Primary antibody: Aldolase A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-Aldolase A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Aldolase A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Aldolase A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Aldolase A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Aldolase A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HeLa cells. Anti- Aldolase A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

ICC shows positive staining in NIH/3T3 cells. Anti-Aldolase A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).