WB result of Aldh5a1 Recombinant Rabbit mAb
Primary antibody: Aldh5a1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-87 MG whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Lane 5: Ramos whole cell lysate 20 µg
Low expression control: U-87 MG whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 55 kDa
Aldh5a1 Recombinant Rabbit mAb (S-4135-76)
Aldh5a1 Recombinant Rabbit mAb (S-4135-76)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Aldh5a1 |
| Synonyms | Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; SSADH; ALDH5A1 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P51649 |
| Clone Number | S-4135-76 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HepG2, Raji, Ramos, mouse brain, mouse liver, rat brain, rat liver, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu |
Background
ALDH5A1 encodes a mitochondrial NAD⁺-dependent succinate semialdehyde dehydrogenase (SSADH), a key enzyme in the catabolic pathway of the neurotransmitter GABA, responsible for the irreversible oxidation of succinate semialdehyde to succinate. Deficiency of this gene leads to a rare autosomal recessive disorder, succinate semialdehyde dehydrogenase deficiency (also known as 4-hydroxybutyric aciduria), in which GABA metabolism is blocked and the neurotoxic metabolite γ-hydroxybutyric acid (GHB) accumulates abnormally, resulting in widespread neurological damage including developmental delay, ataxia, seizures, behavioral disorders, and intellectual disability. Recent studies have revealed that the functional significance of ALDH5A1 extends far beyond its classical metabolic role. In glioblastoma, this gene is significantly downregulated under hypoxic conditions via negative regulation by miR-210, and this downregulation promotes tumor proliferation by reprogramming cellular metabolism and maintaining a high glycolytic phenotype, with low expression closely associated with poor patient prognosis. Conversely, ALDH5A1 overexpression inhibits reactive oxygen species production and shifts cellular metabolism from glycolysis to oxidative phosphorylation, thereby suppressing tumor growth. Additionally, in a mouse model of SSADH deficiency, loss of this enzyme not only impairs GABA metabolism but also leads to astrocyte dysfunction and severe depletion of myelinSphingolipid, suggesting that glial pathology is an important component of the disease mechanism. These findings reposition ALDH5A1 from a conventional metabolic enzyme to a multifunctional regulatory hub linking neurotransmitter metabolism, glial cell function, and tumor metabolic reprogramming.
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Western Blot
WB result of Aldh5a1 Recombinant Rabbit mAb
Primary antibody: Aldh5a1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 50 kDa
WB result of Aldh5a1 Recombinant Rabbit mAb
Primary antibody: Aldh5a1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 50 kDa
WB result of Aldh5a1 Recombinant Rabbit mAb
Primary antibody: Aldh5a1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Aldh5a1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Aldh5a1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Aldh5a1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-Aldh5a1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
