WB result of ALDH2 Recombinant Rabbit mAb
Primary antibody: ALDH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: Ramos whole cell lysate 20 µg
Lane 3: SK-BR-3 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 55 kDa
ALDH2 Recombinant Rabbit mAb (S-4173-20)
ALDH2 Recombinant Rabbit mAb (S-4173-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ALDH2 |
| Synonyms | Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDM |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P05091 |
| Clone Number | S-4173-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | THP-1, Ramos, SK-BR-3, HepG2, mouse liver, mouse kidney, rat liver, rat kidney |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1: 1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
Aldehyde dehydrogenase 2 (ALDH2) is a crucial mitochondrial enzyme that serves as the primary catalyst for the oxidation of acetaldehyde, a toxic byproduct of ethanol metabolism, into harmless acetate, thereby playing a pivotal role in alcohol detoxification and cellular protection against oxidative stress. Encoded by the ALDH2 gene on chromosome 12, this homotetrameric protein is particularly significant in East Asian populations, where a common genetic variant (Glu504Lys, known as ALDH2*2) results in a dominant-negative loss of enzymatic activity, leading to the characteristic "Asian flush" syndrome characterized by facial flushing, nausea, and tachycardia upon alcohol consumption due to acetaldehyde accumulation. Beyond its central function in alcohol metabolism, ALDH2 is increasingly recognized for its broader cytoprotective roles, including the detoxification of reactive aldehydes generated during lipid peroxidation, which links its activity to the pathogenesis of various diseases such as cardiovascular disorders, neurodegenerative conditions, and cancer, making it a promising therapeutic target for mitigating tissue damage induced by oxidative stress and ischemia-reperfusion injury.
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Western Blot
WB result of ALDH2 Recombinant Rabbit mAb
Primary antibody: ALDH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 53 kDa
WB result of ALDH2 Recombinant Rabbit mAb
Primary antibody: ALDH2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 54 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-ALDH2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
