WB result of ALDH1A2 Recombinant Rabbit mAb
Primary antibody: ALDH1A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 55 kDa
ALDH1A2 Recombinant Rabbit mAb (S-3569-78)
ALDH1A2 Recombinant Rabbit mAb (S-3569-78)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ALDH1A2 |
| Synonyms | Retinal dehydrogenase 2; RALDH 2; RalDH2; Aldehyde dehydrogenase family 1 member A2 (ALDH1A2); Retinaldehyde-specific dehydrogenase type 2 (RALDH(II)) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | O94788 |
| Clone Number | S-3569-78 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Rt |
| Positive Sample | K562, HeLa, HEK-293, mouse testis, mouse spleen, mouse heart, rat testis, rat kidney |
| Predicted Reactivity | Ck |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
ALDH1A2, also known as retinaldehyde dehydrogenase 2 (RALDH2), is a critical cytosolic enzyme belonging to the aldehyde dehydrogenase superfamily that serves as the primary catalyst for the irreversible oxidation of retinaldehyde into retinoic acid (RA), a potent derivative of vitamin A essential for embryonic development and tissue homeostasis. By tightly regulating local RA concentrations, ALDH1A2 orchestrates fundamental biological processes including anterior-posterior axis patterning, limb bud formation, heart morphogenesis, and the differentiation of various cell lineages through the activation of nuclear retinoic acid receptors (RARs) which modulate gene transcription. Beyond its pivotal role in development, this protein is implicated in adult physiological functions such as immune regulation and tissue repair, while its dysregulation or specific polymorphisms have been linked to congenital malformations, certain cancers where it may influence stemness and drug resistance, and inflammatory conditions, making it a significant target for therapeutic intervention and a key biomarker in developmental biology and pathology research.
Picture
Picture
Western Blot
WB result of ALDH1A2 Recombinant Rabbit mAb
Primary antibody: ALDH1A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 55 kDa
WB result of ALDH1A2 Recombinant Rabbit mAb
Primary antibody: ALDH1A2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 55 kDa
Immunocytochemistry
ICC shows positive staining in K562 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-ALDH1A2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
