ALCAM Recombinant Rabbit mAb (S-4080-40)

Catalog Number: S0B6928 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25µl 100µl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	ALCAM
Synonyms	CD166 antigen; Activated leukocyte cell adhesion molecule; CD166; MEMD
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	Q13740
Clone Number	S-4080-40
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	JAR, J774A.1, THP-1, SH-SY5Y, HuT-78, Daudi, mouse brain, mouse liver, rat brain, rat liver
Predicted Reactivity	Bv, Rb
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt

Background

CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is encoded by the ALCAM gene and is involved in both homotypic and heterotypic cell adhesion. CD166 is expressed on various cell types, including activated T cells, epithelial cells, fibroblasts, and neurons. It plays a crucial role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during T cell development. Additionally, CD166 is overexpressed in several types of tumors and serves as a potential cancer stem cell marker, making it a target for cancer research and therapy.

Picture

Western Blot

WB result of ALCAM Recombinant Rabbit mAb
Primary antibody: ALCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: JAR whole cell lysate 20 µg
Lane 2: J774A.1 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Lane 5: HuT-78 whole cell lysate 20 µg
Lane 6: Daudi whole cell lysate 20 µg
Negative control: JAR whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 100-130 kDa

WB result of ALCAM Recombinant Rabbit mAb
Primary antibody: ALCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 80-110 kDa

WB result of ALCAM Recombinant Rabbit mAb
Primary antibody: ALCAM Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 80-110 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human stomach. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-ALCAM antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.