AKT1S1/PRAS40 Recombinant Rabbit mAb (S-4240-49)

Catalog Number: S0B60317 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl 1ml

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Product Details

Product Specification

Host	Rabbit
Antigen	AKT1S1/PRAS40
Synonyms	Proline-rich AKT1 substrate 1; PRAS40 s
Immunogen	Synthetic Peptide
Location	Cytoplasm
Accession	Q96B36
Clone Number	S-4240-49
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	293T, MCF7, HeLa, A549, SH-SY5Y, A204, RD, NIH/3T3, RAW264.7, mouse liver, PC-12, C6, rat liver, COS7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	Hu, Ms, Rt, Mk
IHC-P	1:2000	Hu, Ms

Background

AKT1S1, also commonly known as PRAS40 (proline-rich AKT substrate of 40 kDa), serves as a critical "molecular brake" within cells. Its core function is to act as an inhibitory component of the mTORC1 signaling complex, precisely regulating cell growth and metabolism. In the absence of nutrient signals, AKT1S1 binds to the mTORC1 complex, directly blocking the substrate-binding site and thereby suppressing mTORC1 kinase activity, preventing energy-consuming processes such as protein synthesis. Upon stimulation by insulin or growth factors, upstream AKT kinase phosphorylates AKT1S1 at threonine 246 (Thr-246). The phosphorylated AKT1S1 then binds to 14-3-3 proteins and dissociates from the mTORC1 complex, effectively "releasing the brake" to activate mTORC1 signaling and promote cell growth and proliferation. As a key node connecting the PI3K-AKT and mTORC1 signaling pathways, dysregulation of AKT1S1 function is closely associated with various diseases, including cancer and metabolic disorders, and it also plays important roles in stage-specific regulation of normal physiological processes such as spermatogenesis.

Picture

Western Blot

WB result of AKT1S1/PRAS40 Recombinant Rabbit mAb
Primary antibody: AKT1S1/PRAS40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Lane 5: SH-SY5Y whole cell lysate 20 µg
Lane 6: A204 whole cell lysate 20 µg
Lane 7: RD whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 40 kDa

WB result of AKT1S1/PRAS40 Recombinant Rabbit mAb
Primary antibody: AKT1S1/PRAS40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW 264.7 whole cell lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 38 kDa

WB result of AKT1S1/PRAS40 Recombinant Rabbit mAb
Primary antibody: AKT1S1/PRAS40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Lane 3: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 38 kDa

WB result of AKT1S1/PRAS40 Recombinant Rabbit mAb
Primary antibody: AKT1S1/PRAS40 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 38 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-AKT1S1/PRAS40 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human heart muscle. Anti-AKT1S1/PRAS40 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-AKT1S1/PRAS40 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-AKT1S1/PRAS40 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-AKT1S1/PRAS40 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse heart muscle. Anti-AKT1S1/PRAS40 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.