IHC shows positive staining in paraffin-embedded human testis. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
AKAP4 Recombinant Rabbit mAb (SDT-4196)
AKAP4 Recombinant Rabbit mAb (SDT-4196)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AKAP4 |
| Synonyms | A-kinase anchor protein 4; AKAP-4; A-kinase anchor protein 82 kDa (AKAP 82; hAKAP82); Major sperm fibrous sheath protein (HI); Protein kinase A-anchoring protein 4 (PRKA4); AKAP82 |
| Location | Cell projection |
| Accession | Q5JQC9 |
| Clone Number | SDT-4196 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:2000 | Hu |
| IF | 1:500 | Hu |
Background
AKAP4, the most abundant protein of the sperm fibrous sheath, is a testis-enriched scaffolding protein that simultaneously binds PKA via dual-specificity anchoring sites and PKC through polybasic motifs, thereby acting as a cAMP/PKA ↔ PKC/ERK1/2 switch that is phosphorylated on Ser/Tyr during capacitation to direct the kinase cascade to the flagellar principal piece, mid-piece and post-acrosomal region, enabling progressive motility and male fertility while also moonlighting in multiple cancers where its cytoplasmic/membrane accumulation drives proliferation, EMT and metastasis through the Shh and Wnt/β-catenin pathways.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human endometrial cancer. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung cancer. Anti-AKAP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human testis. Anti-AKAP4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
