WB result of AK1C3 Rabbit Polyclonal Antibody
Primary antibody: AK1C3 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: Hep G2 whole cell lysate 20 µg
Lane 4: TF-1 whole cell lysate 20 µg
Negative control: HCT 116 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
AK1C3 Rabbit Polyclonal Antibody
AK1C3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AK1C3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P42330 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | A549, HepG2, TF-1, mouse colon, mouse liver, rat colon, rat liver, COS-7 |
| Predicted Reactivity | Or |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200-1:1000 | Hu |
| IF | 1:500 | Hu |
Background
AK1C3 (full name Aldo-keto reductase family 1 member C3, gene name AKR1C3) is a human cytosolic NADPH-dependent oxidoreductase belonging to the aldo-keto reductase superfamily and composed of 323 amino acids. It plays a central role in the metabolism of steroid hormones and prostaglandins, possessing 3α-, 17β-, and 20α-hydroxysteroid dehydrogenase activities that enable it to catalyze the bidirectional interconversion between ketones and alcohols. Specifically, the most notable catalytic function of AK1C3 is the efficient reduction of androstenedione to testosterone; it can also convert estrone to estradiol, reduce prostaglandin D2 to 11β-prostaglandin F2α, and convert the potent androgen dihydrotestosterone (DHT) to the less active 3α-androstanediol, thereby regulating the local availability of androgens, estrogens, and progestogens. The protein is encoded by a gene located at chromosome 10p15.1 and is expressed in a variety of tissues. Dysregulation of its expression is closely associated with the development and progression of multiple malignant tumors, including prostate cancer, breast cancer, lung cancer, and renal cell carcinoma, making it a potential target of considerable interest in cancer therapy research.
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Western Blot
WB result of AK1C3 Rabbit Polyclonal Antibody
Primary antibody: AK1C3 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse colon lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
WB result of AK1C3 Rabbit Polyclonal Antibody
Primary antibody: AK1C3 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat colon lysate 20 µg
Lane 3: rat liver lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
WB result of AK1C3 Rabbit Polyclonal Antibody
Primary antibody: AK1C3 Rabbit Polyclonal Antibody at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-AK1C3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-AK1C3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cardiac muscle. Anti-AK1C3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-AK1C3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-AK1C3 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon. Anti-AK1C3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human liver. Anti-AK1C3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows negative staining in paraffin-embedded human colon. Anti-AK1C3 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
