WB result of AICDA Rabbit mAb
Primary antibody: AICDA Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Ramos whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDa
AICDA Recombinant Rabbit mAb (S-1030-15)
AICDA Recombinant Rabbit mAb (S-1030-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase (AID), Cytidine aminohydrolase, AID |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q9GZX7 |
| Clone Number | S-1030-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Predicted Reactivity | Bv, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC-P | 1:100 |
Background
The AICDA protein, also known as Activation-Induced Cytidine Deaminase (AID), plays a crucial role in the immune system. As an enzyme, it mediates affinity maturation and promotes DNA demethylation in germinal center B cells. Specifically, AICDA is essential for generating antibody diversity and forming immune memory by inducing mutations and recombination in antibody genes. It facilitates somatic hypermutation and class switch recombination in immunoglobulin genes, thus enhancing the diversity and specificity of antibodies. However, abnormal expression of AICDA has also been associated with certain diseases. For instance, overexpression of AICDA has been shown to render BCL2-driven mouse lymphomas more aggressive. Additionally, excessive expression of AICDA is considered an important source of epigenetic heterogeneity in diffuse large B-cell lymphoma (DLBCL), which is primarily associated with relative loss of cytosine methylation, increasing tumor heterogeneity and overall adaptive capacity.
Picture
Picture
Western Blot
IP
AICDA Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating AICDA in 0.4 mg Daudi whole cell lysate.
Western blot was performed on the immunoprecipitate using AICDA Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Daudi whole cell lysate 20 µg (Input)
Lane 2: AICDA Rabbit mAb IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
Predicted MW: 24 kDa
Observed MW: 24 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-AICDA antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
