Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-20) MediumPlus

Instructions for use

Taking the colon cancer organoid medium configuration as a reference: To 500 ml of organoid basal medium 20, add: R-spondin1 (abs04398-50ug), Noggin (abs04695-50ug), EGF (abs05152-100ug), FGF10, Glutamax (abs9295-100mL), P/S (abs9244-100mL), HEPES (abs42150994-100mL), N-Acetylcysteine (abs822057-10mg), Nicotinamide (abs816969-200mg), A8301 (abs817694-25mg), Gastrin (abs814916-1mg), SB202190 (abs810009-50mg), Y27632 (abs812864-50mg), Can be directly used for intestinal cancer organoid culture.

Product Advantage

• Specially developed basal medium for organoid culture, produced in accordance with GMP regulations, with stable process and small batch benefit.
• Effectively increases the cell proliferation rate under serum-reduced culture without affecting cell viability and morphology.
• All raw materials are selected from self-produced or strictly tested and purified low-endotoxin high-purity medicinal grade raw materials, which have low pyrogen levels and reduce the interference of exogenous endotoxins.

Organoid Basal Medium Plus is a serum-reduced basal medium containing B27 and N2, increasing related protein factors and inhibitors such as R-spondin1, EGF, and Y27632, which can be directly used for various organoid cultures.

Organoid basal medium F20 was supplemented with: B27, N2, insulin, transferrin, bovine serum albumin, ethanolamine, ascorbic acid, glutathione, bispecific antibodies, and various trace elements (sodium selenite, ammonium metavanadate, manganese chloride, and copper sulfate).

-20℃, shelf life 1 year.